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In vitro requirements of temperature and humidity in the environment In modern times, not all plants germinate and grow through seeds. Nowadays, many plants are cultivated by sectioning on the basis of their original branches. A section of it calls it an in vitro plant. The environmental requirements of the plant in vitro, the temperature and humidity in the environment, and the light intensity in the environment will all have a certain influence. We can measure and use it professionally. For regulation, temperature and humidity measurements can be made using a temperature and humidity monitor, and light intensity measurements can be performed using an optical quantum meter. Here's a look at the effects of temperature and humidity in the environment on ex-plants.
The isolated material of the plant must meet its requirements for air humidity. After the plant material is cut off from the mother, the moisture can no longer be absorbed from the mother's source, moisture absorption and transpiration are broken, and the water metabolism is unbalanced. The ex-plant material of the seedbed reduces transpiration and also provides air humidity in the micro-environment surrounding the leaf, especially during the first phase of rapid propagation, the transpiration of the ex-vivo material is strong, and the incision has not yet absorbed moisture, if the air humidity If it is too low and transpiration is too prosperous, water loss and desiccation will lead to failure of rapid propagation. Therefore, the air humidity in the micro-environment of the first stage leaf must be maintained at 90% or even 100%.
The development of plant material in vitro needs to meet its basic temperature requirements. The two factors that affect the material development are the two temperature limits. One is the low temperature limit, and the other is the high temperature limit. The low limit of the expert system operation is set to 15°C. When the intelligent blade senses seedbed temperature below 15°C, the air heating system is automatically turned on to warm the air. High temperature has great influence on the development of isolated materials. When the leaf temperature of most plants exceeds 35°C, respiration is enhanced, photosynthesis is decreased, and even the products consumed by respiration exceed the photosynthetic accumulation and cause high-temperature persecution, and carbon sources cannot be provided for rooting. Hormones, thus affecting rooting. Therefore, the effective control of high temperature is a key factor affecting rapid propagation, which is more important than the control of low temperature limit. Especially in the rapid propagation of high temperature season, the important parameter affecting rapid propagation is high temperature.
Light is the energy of plant photosynthesis. Without sunlight, photosynthesis cannot be carried out, and the formation and development of adventitious roots will be seriously affected. In general, the light is sufficient, the photosynthesis is vigorous, the formation of carbohydrates is more, and the material accumulation in the ex vivo material is more. The origin and development of roots are robust, so light is the source of material for rooting of in vitro materials. However, it is not necessarily the case that the stronger the light is, the better, and the strong light will produce the phenomenon of photo-suppression, which is unfavorable for rooting. Therefore, it is more important to control the light scientifically.
When cultivating ex vivo plants, it is best performed in an incubator because the above humidity is not well achieved in the external environment, but it is much easier in the incubator.
The isolated material of the plant must meet its requirements for air humidity. After the plant material is cut off from the mother, the moisture can no longer be absorbed from the mother's source, moisture absorption and transpiration are broken, and the water metabolism is unbalanced. The ex-plant material of the seedbed reduces transpiration and also provides air humidity in the micro-environment surrounding the leaf, especially during the first phase of rapid propagation, the transpiration of the ex-vivo material is strong, and the incision has not yet absorbed moisture, if the air humidity If it is too low and transpiration is too prosperous, water loss and desiccation will lead to failure of rapid propagation. Therefore, the air humidity in the micro-environment of the first stage leaf must be maintained at 90% or even 100%.
The development of plant material in vitro needs to meet its basic temperature requirements. The two factors that affect the material development are the two temperature limits. One is the low temperature limit, and the other is the high temperature limit. The low limit of the expert system operation is set to 15°C. When the intelligent blade senses seedbed temperature below 15°C, the air heating system is automatically turned on to warm the air. High temperature has great influence on the development of isolated materials. When the leaf temperature of most plants exceeds 35°C, respiration is enhanced, photosynthesis is decreased, and even the products consumed by respiration exceed the photosynthetic accumulation and cause high-temperature persecution, and carbon sources cannot be provided for rooting. Hormones, thus affecting rooting. Therefore, the effective control of high temperature is a key factor affecting rapid propagation, which is more important than the control of low temperature limit. Especially in the rapid propagation of high temperature season, the important parameter affecting rapid propagation is high temperature.
Light is the energy of plant photosynthesis. Without sunlight, photosynthesis cannot be carried out, and the formation and development of adventitious roots will be seriously affected. In general, the light is sufficient, the photosynthesis is vigorous, the formation of carbohydrates is more, and the material accumulation in the ex vivo material is more. The origin and development of roots are robust, so light is the source of material for rooting of in vitro materials. However, it is not necessarily the case that the stronger the light is, the better, and the strong light will produce the phenomenon of photo-suppression, which is unfavorable for rooting. Therefore, it is more important to control the light scientifically.
When cultivating ex vivo plants, it is best performed in an incubator because the above humidity is not well achieved in the external environment, but it is much easier in the incubator.